Division of Crop Improvement

Major Areas of Research

  • Germplasm collection, evaluation and maintenance
  • Breeding of vegetable varieties for premium traits
  • Mapping and tagging of genes for useful traits
  • Development of transgenic lines
  • DNA fingerprinting of vegetable varieties
  • Hybrid seed purity testing
  • Studies of seed-transmitted pathogens
  • Nuclear and breeder seed production

Facilities Avaiablae

  • Molecular biology laboratory
  • Genomics laboratory
  • Plant tissue culture room
  • Seed technology laboratory
  • DNA sequencer
  • DNA microarray
  • Protein purification system
  • PCR Tetrad
  • ELISA reader
  • Automated gel electrophoresis system
  • Liquid Nitrogen generator
  • Gel documentation system
  • Fluorescence microscope
  • Inverted microscope

 


Grmplasm Status

Crop

Total number

Crop

Total number

Tomato

1200

Bottle gourd

195

Brinjal

440

Pointed gourd

180

Chilli

350

Ivy gourd

35

Okra

374

Pumpkin

178

Pea

435

Ash gourd

74

Cauliflower

182

Muskmelon

100

Radish

76

Cucumber

200

Carrot

44

Ridge gourd

34

Cowpea

268

Sponge gourd

43

French bean

244

Bitter gourd

320

Dolichos bean

320

 

 

TOTAL

 

5292

 

 


Grmplasm registered for their unique traits

Crop

Genotype

Trait

Registration number

Bitter gourd

Gy263B

Gynoceism

INGR No. 03037

Pointed gourd

IIVRPG-105

Parthenocarpy

INGR No. 03035

Chilli

BS35

PepLCV resistance

INGR No. 07039

Okra

No.315

Dwarf and short internodes

INGR No. 05026

Pumpkin

SA90

High carotenoids

INGR No. 05027

Snap melon

B159

Downy mildew resistance

INGR No. 07044

Tomato

F6050

Joint-less peduncle

INGR No. 06036

Tomato

F7028

High lycopene content

INGR No. 06037

 


Varieties/hybrids devloped (click here)


Research Achievements

 

  • Gene expression profiling for drought tolerance were done using EC520061 as tolerant and CO-3 as susceptible line, 165 genes found exclusively up-regulated in tolerant plants against drought stress, while 380 genes were found up –regulated in susceptible plants, The genes from different pathways responsible for abiotic stress tolerance were selected for further proteomics studies.

  • Gene expression profiling for heat tolerance were done using PS-1 as tolerant and H-24 as susceptible line, 32 genes found exclusively up-regulated in tolerant plants against heat stress, while 244 genes were found up–regulated in susceptible plants.

  • Six varieties of brinjal have been transformed with Cry1Ac through back cross for fruit and shoot borer (FSB) resistance.

  • Fruit and shoot borer (EFSB) resistant transgenic line of brinjal var. IVBL-9 has been developed utilizing Cry1Ac gene by Agrobacterium-mediated transformation. The lines are in T5 generation for event selection.

  • Fruit borer resistant transgenic line of Tomato var. H-86 has been developed utilizing Cry1Ac gene by Agrobacterium-mediated transformation. The lines are in T5 generation for event selection.

  • In brinjal, RAPD markers viz., OPE022000 for hybrid Kashi Sandesh, OPJ121000 for hybrid Kashi Komal, and OPU191700 for hybrid VR-3 have been found to be male parent specific, and are being used for hybrid seed purity testing.

  • Three RAPD markers, OPC051000 (male sex associated), OPC14400 (female sex associated) and OPN011030 (absent in parthenocarpic line) have been identified, which together can reliably identify the staminate, pistillate, and parthenocarpic types of T. dioica.

  •  In-vitro multiplication protocol has been developed for Trichosanthes for large scale multiplication of elite materials.

  • H-88-78-1 and EC-520861 of tomato were identified as resistant source for both early blight and TLCV.

  • Cytoplasmic and nuclear male sterile lines have been developed in chilli, which is being utilized in developing cost effective hybrids

  • Two previously known RAPD markers (OPW19800 and OPP131400) associated with Rf gene were tested in 47 restorer and maintainer lines of Capsicum annuum (chilli and sweet pepper). Both these markers were found useful for genetic purity testing of cms-based commercial hybrid CCH-2.

  • In bitter gourd, gynoecious line (Gy263B) was identified and characterized, which is expected to be of immense use in heterosis breeding. Inheritance of gynoecism was examined and found to be under the control of single recessive gene.

  • Petha Preparaption technology has been standardized and suitable variety and hybrids of ash gourd has been recommended for petha preparation. Protocol of by-product i.e. Vari and Tilori has been standardized.

  • Priming of carrot seeds with 150 ppm KNO3 retained its germination for a year as compared to other treatments and control.